Channelrhodopsin-1: A Light-Gated Proton Channel in Green Algae

Author:

Nagel Georg1,Ollig Doris1,Fuhrmann Markus2,Kateriya Suneel2,Musti Anna Maria3,Bamberg Ernst1,Hegemann Peter2

Affiliation:

1. Max-Planck-Institut für Biophysik, Kennedyallee 70, 60596 Frankfurt am Main, Germany.

2. Institut für Biochemie, Universität Regensburg, Universitätsstrasse 31, 93040 Regensburg, Germany.

3. Dipartimento Farmaco-Biologico Università della Calabria, 87036 Rende, Italy.

Abstract

Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all- trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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