Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region-Reference-Cited by-同舟云学术

Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region

Published:2018-11-16 Issue:6416 Volume:362 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Moffitt Jeffrey R.¹²³⁴^ORCID,Bambah-Mukku Dhananjay¹⁴⁵^ORCID,Eichhorn Stephen W.¹²³⁴^ORCID,Vaughn Eric¹⁴⁵,Shekhar Karthik⁶,Perez Julio D.¹⁴⁵^ORCID,Rubinstein Nimrod D.¹⁴⁵,Hao Junjie¹²³⁴,Regev Aviv¹⁶⁷^ORCID,Dulac Catherine¹⁴⁵^ORCID,Zhuang Xiaowei¹²³⁴^ORCID

Affiliation:

1. Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.

2. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

3. Department of Physics, Harvard University, Cambridge, MA 02138, USA.

4. Center for Brain Science, Harvard University, Cambridge, MA 02138, USA.

5. Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

6. Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA 02139, USA.

7. Koch Institute of Integrative Cancer Biology, Department of Biology, MIT, Cambridge, MA 02139, USA.

Abstract

Mapping the brain, one neuron at a time Spatial transcriptomics can link molecularly described cell types to their anatomical positions and functional roles. Moffitt et al. used a combination of single-cell RNA-sequencing and MERFISH (multiplexed error-robust fluorescence in situ hybridization) to map the identity and location of specific cell types within the mouse preoptic hypothalamus and surrounding areas of the brain (see the Perspective by Tasic and Nicovich). They related these cell types to specific behaviors via gene activity. The approach provides an unbiased description of cell types of the preoptic area, which are important for sleep, thermoregulation, thirst, and social behavior. Science , this issue p. eaau5324 ; see also p. 749

Funder

National Institutes of Health

Howard Hughes Medical Institute

National Institute of Mental Health

National Institute of Child Health and Human Development

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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