Structure of monkeypox virus DNA polymerase holoenzyme-Reference-Cited by-同舟云学术

Structure of monkeypox virus DNA polymerase holoenzyme

Published:2023-01-06 Issue:6627 Volume:379 Page:100-105
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Peng Qi¹^ORCID,Xie Yufeng²^ORCID,Kuai Lu¹^ORCID,Wang Han¹³,Qi Jianxun¹^ORCID,Gao George F.¹²⁴⁵⁶^ORCID,Shi Yi¹⁵⁶^ORCID

Affiliation:

1. CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

2. Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China.

3. College of Future Technology, Peking University, Beijing 100871, China.

4. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China.

5. Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Disease (CEEID), Chinese Academy of Sciences, Beijing 100101, China.

6. Research Unit of Adaptive Evolution and Control of Emerging Viruses, Chinese Academy of Medical Sciences, Beijing 100052, China.

Abstract

The World Health Organization declared mpox (or monkeypox) a public health emergency of international concern in July 2022, and prophylactic and therapeutic measures are in urgent need. The monkeypox virus (MPXV) has its own DNA polymerase F8, together with the processive cofactors A22 and E4, constituting the polymerase holoenzyme for genome replication. Here, we determined the holoenzyme structure in complex with DNA using cryo–electron microscopy at the global resolution of ~2.8 angstroms. The holoenzyme possesses an architecture that suggests a “forward sliding clamp” processivity mechanism for viral DNA replication. MPXV polymerase has a DNA binding mode similar to that of other B-family DNA polymerases from different species. These findings reveal the mechanism of the MPXV genome replication and may guide the development of anti-poxvirus drugs.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference53 articles.

1. The first imported case of monkeypox in the mainland of China-Chongqing municipality, China, September 16, 2022;Zhao H.;China CDC Wkly.,2022

2. Prevention and Treatment of Monkeypox

3. Monkeypox: A Comprehensive Review of Transmission, Pathogenesis, and Manifestation

4. Monkeypox: An international epidemic

5. Human monkeypox: an emerging zoonosis

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