Real-Time Dynamics of RNA Polymerase II Clustering in Live Human Cells-Reference-Cited by-同舟云学术

Real-Time Dynamics of RNA Polymerase II Clustering in Live Human Cells

Published:2013-08-09 Issue:6146 Volume:341 Page:664-667
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Cisse Ibrahim I.¹²³,Izeddin Ignacio¹²,Causse Sebastien Z.¹,Boudarene Lydia¹²,Senecal Adrien¹⁴,Muresan Leila⁵,Dugast-Darzacq Claire¹⁶,Hajj Bassam³,Dahan Maxime²³⁴,Darzacq Xavier¹³

Affiliation:

1. Functional Imaging of Transcription, CNRS UMR8197, Ecole Normale Supérieure, Institut de Biologie de l’ENS, IBENS, Paris, 75005 France.

2. Laboratoire Kastler Brossel, CNRS UMR8552, Département de Physique, Institut de Biologie de l'Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris cedex 05, France.

3. Transcription Imaging Consortium, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.

4. Université Pierre et Marie Curie—Paris 6, 75005 Paris, France.

5. DYOGEN, CNRS, UMR8197, Ecole Normale Supérieure, Institut de Biologie de l’ENS, IBENS, Paris, 75005 France.

6. Université Paris Diderot, Paris Cité Sorbonne, 75013 Paris, France.

Abstract

Pol II Micro Clusters In higher eukaryotes, messenger RNA (mRNA) synthesis is thought to involve foci of clustered RNA polymerase II (Pol II) called transcription factories. However, clustered Pol II have not been resolved in living cells, raising the debate about their existence in vivo and what role, if any, they play in nuclear organization and regulation of gene expression. Cisse et al. (p. 664 , published online 4 July; see the Perspective by Rickman and Bickmore ) developed single-molecule in vivo analyses revealing the distribution and dynamics of Pol II clustering in living cells. Pol II clusters were smaller than the diffraction limit (<250 nm). Transient dynamics of the Pol II clusters, and correlation with changes in transcription, pointed to a role in transcription initiation rather than in elongation.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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