1. Our library of compounds consists of a diverse set of 10 000 small molecules (average molecular weight 213) dissolved in perdeuterated dimethylsulfoxide (DMSO).
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4. A two-dimensional (2D) HSQC spectrum of a uniformly 15 N-labeled protein can be acquired in about 10 minutes on a 500-MHz NMR spectrometer allowing 100 2D spectra to be recorded in 1 day with an automatic sample changer. If compounds are tested in mixtures of 10 (as in our strategy) it is possible to robotically screen 1000 compounds per day.
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