The use of ectopic volar fibroblasts to modify skin identity-Reference-Cited by-同舟云学术

The use of ectopic volar fibroblasts to modify skin identity

Published:2024-09-06 Issue:6713 Volume:385 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Lee Sam S.¹^ORCID,Sweren Evan¹^ORCID,Dare Erika¹^ORCID,Derr Paige²,Derr Kristy²,Wang Chen Chia¹^ORCID,Hardesty Brooke¹^ORCID,Willis Aiden A.¹^ORCID,Chen Junjie³^ORCID,Vuillier Jonathan K.¹^ORCID,Du Joseph¹^ORCID,Wool Julia¹^ORCID,Ruci Amanda¹^ORCID,Wang Vicky Y.¹^ORCID,Lee Chaewon¹^ORCID,Iyengar Sampada¹^ORCID,Asami Soichiro⁴^ORCID,Daskam Maria¹^ORCID,Lee Claudia¹,Lee Jeremy C.¹^ORCID,Cho Darren¹^ORCID,Kim Joshua¹,Martinez-Peña Eddie Gibson¹^ORCID,Lee So Min¹^ORCID,He Xu¹,Wakeman Michael¹,Sicilia Iralde¹,Dobbs Dalhart T.¹^ORCID,van Ee Amy¹^ORCID,Li Ang¹^ORCID,Xue Yingchao¹^ORCID,Williams Kaitlin L.¹^ORCID,Kirby Charles S.¹^ORCID,Kim Dongwon¹^ORCID,Kim Sooah¹^ORCID,Xu Lillian¹^ORCID,Wang Ruizhi¹,Ferrer Marc²,Chen Yun³^ORCID,Kang Jin U.⁵^ORCID,Kalhor Reza⁴^ORCID,Kang Sewon¹^ORCID,Garza Luis A.¹⁶⁷^ORCID

Affiliation:

1. Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

2. Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA.

3. Department of Mechanical Engineering, Johns Hopkins University, MD 21210, USA.

4. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

5. Department of Electrical and Computer Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

6. Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

7. Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

Abstract

Skin identity is controlled by intrinsic features of the epidermis and dermis and their interactions. Modifying skin identity has clinical potential, such as the conversion of residual limb and stump (nonvolar) skin of amputees to pressure-responsive palmoplantar (volar) skin to enhance prosthesis use and minimize skin breakdown. Greater keratin 9 ( KRT9 ) expression, higher epidermal thickness, keratinocyte cytoplasmic size, collagen length, and elastin are markers of volar skin and likely contribute to volar skin resiliency. Given fibroblasts’ capacity to modify keratinocyte differentiation, we hypothesized that volar fibroblasts influence these features. Bioprinted skin constructs confirmed the capacity of volar fibroblasts to induce volar keratinocyte features. A clinical trial of healthy volunteers demonstrated that injecting volar fibroblasts into nonvolar skin increased volar features that lasted up to 5 months, highlighting a potential cellular therapy.

Publisher

American Association for the Advancement of Science (AAAS)

Link

https://www.science.org/doi/pdf/10.1126/science.adi1650

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