An electron transfer path connects subunits of a mycobacterial respiratory supercomplex

Author:

Gong Hongri1ORCID,Li Jun23ORCID,Xu Ao14ORCID,Tang Yanting1ORCID,Ji Wenxin45ORCID,Gao Ruogu45,Wang Shuhui23ORCID,Yu Lu6ORCID,Tian Changlin67,Li Jingwen8,Yen Hsin-Yung89,Man Lam Sin10,Shui Guanghou10,Yang Xiuna23,Sun Yuna4,Li Xuemei4,Jia Minze4,Yang Cheng1ORCID,Jiang Biao2,Lou Zhiyong11,Robinson Carol V.8ORCID,Wong Luet-Lok12ORCID,Guddat Luke W.13,Sun Fei45ORCID,Wang Quan4ORCID,Rao Zihe123411ORCID

Affiliation:

1. State Key Laboratory of Medicinal Chemical Biology and College of Life Science, Nankai University, Tianjin 300353, China.

2. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China.

3. CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), 320 Yueyang Road, Shanghai 200031, China.

4. National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, CAS, Beijing 100101, China.

5. University of Chinese Academy of Sciences, Beijing, China.

6. High Magnetic Field Laboratory, CAS, Hefei 230031, China.

7. Hefei National Laboratory of Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.

8. Department of Chemistry, University of Oxford, Physical and Theoretical Chemistry Laboratory, South Parks Rd, Oxford, OX1 3QZ, UK.

9. OMass Technologies, Begbroke Science Park, Woodstock Rd, Yarnton, Kidlington OX5 1PF, UK.

10. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China.

11. Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.

12. Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford OX1 3QR, UK.

13. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, 4072 Queensland, Australia.

Abstract

We report a 3.5-angstrom-resolution cryo–electron microscopy structure of a respiratory supercomplex isolated from Mycobacterium smegmatis. It comprises a complex III dimer flanked on either side by individual complex IV subunits. Complex III and IV associate so that electrons can be transferred from quinol in complex III to the oxygen reduction center in complex IV by way of a bridging cytochrome subunit. We observed a superoxide dismutase-like subunit at the periplasmic face, which may be responsible for detoxification of superoxide formed by complex III. The structure reveals features of an established drug target and provides a foundation for the development of treatments for human tuberculosis.

Funder

National Natural Science Foundation of China

Strategic Priority Research Program of the Chinese Academy of Sciences

National Key Research and Development Program of China

State Key Development Program for Basic Research of the Ministry of Science and Technology of China

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Cited by 119 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3