Visualization of cellulose synthases in Arabidopsis secondary cell walls

Author:

Watanabe Y.12,Meents M. J.12,McDonnell L. M.2,Barkwill S.2,Sampathkumar A.3,Cartwright H. N.4,Demura T.5,Ehrhardt D. W.46,Samuels A.L.1,Mansfield S. D.2

Affiliation:

1. Department of Botany, University of British Columbia, Vancouver, BC, Canada.

2. Department of Wood Science, University of British Columbia, Vancouver, BC, Canada.

3. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

4. Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA.

5. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan.

6. Department of Biological Sciences, Stanford University, Stanford, CA, USA.

Abstract

Secondary cell walls built with speed Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science , this issue p. 198 , see also p. 156

Funder

NSF

Canadian Natural Sciences and Engineering Research Council

Japan Society for the Promotion of Science KAKENHI

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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