Recovery of Replication-Competent HIV Despite Prolonged Suppression of Plasma Viremia-Reference-Cited by-全球学者库

Recovery of Replication-Competent HIV Despite Prolonged Suppression of Plasma Viremia

Published:1997-11-14 Issue:5341 Volume:278 Page:1291-1295
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Wong Joseph K.¹²³,Hezareh Marjan¹²³,Günthard Huldrych F.¹²³,Havlir Diane V.¹²³,Ignacio Caroline C.¹²³,Spina Celsa A.¹²³,Richman Douglas D.¹²³

Affiliation:

1. J. K. Wong, M. Hezareh, H. F. Günthard, C. C. Ignacio, C. A. Spina, Departments of Medicine and Pathology, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA.

2. D. V. Havlir, Departments of Medicine and Pathology, University of California, San Diego, School of Medicine, 9500 GIlman Drive, La Jolla, CA 92093, USA, and Treatment Center, University of California, San Diego, 2760 Fifth Avenue, Suite 300, San Diego, CA 92103, USA.

3. D. D. Richman, Departments of Medicine and Pathology, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA, and Veterans Affairs San Diego Healthcare System, San Diego, CA 92063, USA.

Abstract

In evaluating current combination drug regimens for treatment of human immunodeficiency virus (HIV) disease, it is important to determine the existence of viral reservoirs. After depletion of CD8 cells from the peripheral blood mononuclear cells (PBMCs) of both patients and normal donors, activation of patient CD4 lymphocytes with immobilized antibodies to CD3 and CD28 enabled the isolation of virus from PBMCs of six patients despite the suppression of their plasma HIV RNA to fewer than 50 copies per milliliter for up to 2 years. Partial sequencing of HIV pol revealed no new drug resistance mutations or discernible evolution, providing evidence for viral latency rather than drug failure.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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