Environmental Genome Shotgun Sequencing of the Sargasso Sea-Reference-Cited by-同舟云学术

Environmental Genome Shotgun Sequencing of the Sargasso Sea

Published:2004-04-02 Issue:5667 Volume:304 Page:66-74
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Venter J. Craig¹²³⁴⁵,Remington Karin¹²³⁴⁵,Heidelberg John F.¹²³⁴⁵,Halpern Aaron L.¹²³⁴⁵,Rusch Doug¹²³⁴⁵,Eisen Jonathan A.¹²³⁴⁵,Wu Dongying¹²³⁴⁵,Paulsen Ian¹²³⁴⁵,Nelson Karen E.¹²³⁴⁵,Nelson William¹²³⁴⁵,Fouts Derrick E.¹²³⁴⁵,Levy Samuel¹²³⁴⁵,Knap Anthony H.¹²³⁴⁵,Lomas Michael W.¹²³⁴⁵,Nealson Ken¹²³⁴⁵,White Owen¹²³⁴⁵,Peterson Jeremy¹²³⁴⁵,Hoffman Jeff¹²³⁴⁵,Parsons Rachel¹²³⁴⁵,Baden-Tillson Holly¹²³⁴⁵,Pfannkoch Cynthia¹²³⁴⁵,Rogers Yu-Hui¹²³⁴⁵,Smith Hamilton O.¹²³⁴⁵

Affiliation:

1. The Institute for Biological Energy Alternatives, 1901 Research Boulevard, Rockville, MD 20850, USA.

2. The Center for the Advancement of Genomics, 1901 Research Boulevard, Rockville, MD 20850, USA.

3. The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.

4. The J. Craig Venter Science Foundation Joint Technology Center, 5 Research Place, Rockville, MD 20850, USA.

5. University of Southern California, 223 Science Hall, Los Angeles, CA 90089â0740, USA.

Abstract

We have applied âwhole-genome shotgun sequencingâ to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference47 articles.

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5. Materials and Methods are available as supporting online material (SOM) on Science Online.

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