Drosophila Mitotic Domain Boundaries as Cell Fate Boundaries-Reference-Cited by-同舟云学术

Drosophila Mitotic Domain Boundaries as Cell Fate Boundaries

Published:1997-08-08 Issue:5327 Volume:277 Page:825-828
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Cambridge Sidney B.¹²,Davis Robert L.¹²,Minden Jonathan S.¹²

Affiliation:

1. S. B. Cambridge and J. S. Minden, Department of Biological Sciences and Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA.

2. R. L. Davis, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Fate determination in Drosophila embryos is evidenced by the appearance of mitotic domains. To identify fate or fates of cells, individual cells in mitotic domains 2, 8, and 15 were marked and monitored through development. Comparison of the different fates indicated that domain boundaries are cell fate boundaries. Cells were marked by expression of GAL4-dependent transgenes after photoactivation of a caged GAL4VP16 analog that had its DNA binding activity inhibited with a photolabile blocking reagent. Caged GAL4VP16 was also used to induce gene expression in Xenopus embryos. Thus, photoactivated gene expression is a versatile tool for spatiotemporal control of gene expression.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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5. . The final preparations were 75 to 80% pure as judged by SDS–polyacrylamide gel electrophoresis. GAL4VP16 was stored at −80°C in storage buffer [50 mM Na-Hepes 0.2 M NaCl 1 mM dithiothreitol 30% glycerol 0.1 mM EDTA 10 μM Zn acetate (pH 7.4)].

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