Bright and photostable chemigenetic indicators for extended in vivo voltage imaging-Reference-Cited by-同舟云学术

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

Published:2019-08-16 Issue:6454 Volume:365 Page:699-704
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Abdelfattah Ahmed S.¹^ORCID,Kawashima Takashi¹^ORCID,Singh Amrita¹²^ORCID,Novak Ondrej¹³^ORCID,Liu Hui¹^ORCID,Shuai Yichun¹^ORCID,Huang Yi-Chieh⁴^ORCID,Campagnola Luke⁵^ORCID,Seeman Stephanie C.⁵^ORCID,Yu Jianing¹,Zheng Jihong¹,Grimm Jonathan B.¹^ORCID,Patel Ronak¹^ORCID,Friedrich Johannes⁶⁷⁸^ORCID,Mensh Brett D.¹^ORCID,Paninski Liam⁶⁷,Macklin John J.¹,Murphy Gabe J.⁵,Podgorski Kaspar¹,Lin Bei-Jung⁴,Chen Tsai-Wen⁴,Turner Glenn C.¹^ORCID,Liu Zhe¹^ORCID,Koyama Minoru¹^ORCID,Svoboda Karel¹^ORCID,Ahrens Misha B.¹^ORCID,Lavis Luke D.¹^ORCID,Schreiter Eric R.¹^ORCID

Affiliation:

1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

2. Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA.

3. Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

4. Institute of Neuroscience, National Yang-Ming University, Taipei 112, Taiwan.

5. Allen Institute for Brain Science, Seattle, WA 98109, USA.

6. Department of Statistics and Center for Theoretical Neuroscience, Columbia University, New York, NY 10027, USA.

7. Department of Neuroscience and Grossman Center for the Statistics of Mind, Columbia University, New York, NY 10027, USA.

8. Center for Computational Biology, Flatiron Institute, New York, NY 10010, USA.

Abstract

Visualizing neuronal activity in vivo Imaging the changes in fluorescence of voltage-sensitive reagents would enable monitoring of the activity of neurons in vivo. Abdelfattah et al. created such a voltage indicator by designing a protein that combines the voltage sensor domain from microbial rhodopsin with a domain that captures a dye molecule with exceptional brightness and photostability. When the protein was expressed in mice, flies, or zebrafish, they could monitor single action potentials in dozens of neurons simultaneously for many minutes. Science , this issue p. 699

Funder

Howard Hughes Medical Institute

Simons Foundation

Ministry of Science and Technology, Taiwan

IARPA MICrONS

Taiwan National Health Research Institute

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference62 articles.

1. Ultrasensitive fluorescent proteins for imaging neuronal activity