Removal of <i>Pseudomonas</i> type IV pili by a small RNA virus-Reference-Cited by-同舟云学术

Removal of Pseudomonas type IV pili by a small RNA virus

Published:2024-04-05 Issue:6691 Volume:384 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Thongchol Jirapat¹²^ORCID,Yu Zihao¹²^ORCID,Harb Laith¹²^ORCID,Lin Yiruo³,Koch Matthias⁴⁵⁶⁷^ORCID,Theodore Matthew¹²^ORCID,Narsaria Utkarsh¹²,Shaevitz Joshua⁴⁶^ORCID,Gitai Zemer⁵^ORCID,Wu Yinghao⁸,Zhang Junjie¹²^ORCID,Zeng Lanying¹²^ORCID

Affiliation:

1. Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA.

2. Center for Phage Technology, Texas A&M University, College Station, TX 77843, USA.

3. Department of Computer Science and Engineering, Texas A&M University, College Station, TX 77843, USA.

4. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.

5. Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

6. Joseph Henry Laboratories of Physics, Princeton University, Princeton, NJ 08544, USA.

7. Department of Biology, Texas A&M University, College Station, TX 77843, USA.

8. Department of Systems and Computational Biology, Albert Einstein College of Medicine, NY 10461, USA.

Abstract

The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen Pseudomonas aeruginosa . The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through pilus retraction. Using fluorescence microscopy, we discovered that PP7 detaches T4P, which impairs cell motility and restricts the pathogen’s virulence. Using cryo–electron microscopy, mutagenesis, optical trapping, and Langevin dynamics simulation, we resolved the structure of PP7, T4P, and the PP7/T4P complex and showed that T4P detachment is driven by the affinity between the phage maturation protein and its bound pilin, plus the pilus retraction force and speed, and pilus bending. Pilus detachment may be widespread among other ssRNA phages and their retractile pilus systems and offers new prospects for antibacterial prophylaxis and therapeutics.

Publisher

American Association for the Advancement of Science (AAAS)

Link

https://www.science.org/doi/pdf/10.1126/science.adl0635

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