A Virulence Locus of Pseudomonas aeruginosa Encodes a Protein Secretion Apparatus-Reference-Cited by-同舟云学术

A Virulence Locus of Pseudomonas aeruginosa Encodes a Protein Secretion Apparatus

Published:2006-06-09 Issue:5779 Volume:312 Page:1526-1530
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Mougous Joseph D.¹²³⁴⁵,Cuff Marianne E.¹²³⁴⁵,Raunser Stefan¹²³⁴⁵,Shen Aimee¹²³⁴⁵,Zhou Min¹²³⁴⁵,Gifford Casey A.¹²³⁴⁵,Goodman Andrew L.¹²³⁴⁵,Joachimiak Grazyna¹²³⁴⁵,Ordoñez Claudia L.¹²³⁴⁵,Lory Stephen¹²³⁴⁵,Walz Thomas¹²³⁴⁵,Joachimiak Andrzej¹²³⁴⁵,Mekalanos John J.¹²³⁴⁵

Affiliation:

1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.

2. Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

3. Midwest Center for Structural Genomics and Structural Biology Center, Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.

4. Division of Respiratory Diseases and Cystic Fibrosis Center, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.

5. Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.

Abstract

Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts. Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretion apparatus. The apparatus assembled in discrete subcellular locations and exported Hcp1, a hexameric protein that forms rings with a 40 angstrom internal diameter. Regulatory patterns of HSI-I suggested that the apparatus functions during chronic infections. We detected Hcp1 in pulmonary secretions of cystic fibrosis (CF) patients and Hcp1-specific antibodies in their sera. Thus, HSI-I likely contributes to the pathogenesis of P. aeruginosa in CF patients. HSI-I–related loci are widely distributed among bacterial pathogens and may play a general role in mediating host interactions.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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