Ribonucleotide incorporation enables repair of chromosome breaks by nonhomologous end joining-Reference-Cited by-同舟云学术

Ribonucleotide incorporation enables repair of chromosome breaks by nonhomologous end joining

Published:2018-09-14 Issue:6407 Volume:361 Page:1126-1129
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Pryor John M.¹^ORCID,Conlin Michael P.¹^ORCID,Carvajal-Garcia Juan¹^ORCID,Luedeman Megan E.¹^ORCID,Luthman Adam J.¹^ORCID,Small George W.¹^ORCID,Ramsden Dale A.¹^ORCID

Affiliation:

1. Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA.

Abstract

RNA takes over DNA repair Damage to DNA genomes is normally thought to be repaired with DNA. Pryor et al. now describe a clear exception (see the Perspective by Modesti). They found that RNA is routinely incorporated during the repair of DNA double-strand breaks through the mammalian nonhomologous end–joining (NHEJ) pathway. In a variety of contexts, including V(D)J recombination and Cas9-induced genome engineering, two “DNA” polymerases specific to NHEJ preferentially added RNA in cells. These RNA additions facilitated the critical step of ligation and were later replaced by DNA to complete the NHEJ repair process. Science , this issue p. 1126 ; see also p. 1069

Funder

American Cancer Society

National Cancer Institute

National Institute of General Medical Sciences

Publisher

American Association for the Advancement of Science (AAAS)

Link

https://www.science.org/doi/pdf/10.1126/science.aat2477

Reference27 articles.

1. Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants

2. Nonhomologous end joining: A good solution for bad ends

3. Lack of N Regions in Antigen Receptor Variable Region Genes of TdT-Deficient Lymphocytes