Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations-Reference-Cited by-同舟云学术

Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations

Published:2007-04-27 Issue:5824 Volume:316 Page:593-597
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Ishii Nobuyoshi¹²³⁴⁵,Nakahigashi Kenji¹²³⁴⁵,Baba Tomoya¹²³⁴⁵,Robert Martin¹²³⁴⁵,Soga Tomoyoshi¹²³⁴⁵,Kanai Akio¹²³⁴⁵,Hirasawa Takashi¹²³⁴⁵,Naba Miki¹²³⁴⁵,Hirai Kenta¹²³⁴⁵,Hoque Aminul¹²³⁴⁵,Ho Pei Yee¹²³⁴⁵,Kakazu Yuji¹²³⁴⁵,Sugawara Kaori¹²³⁴⁵,Igarashi Saori¹²³⁴⁵,Harada Satoshi¹²³⁴⁵,Masuda Takeshi¹²³⁴⁵,Sugiyama Naoyuki¹²³⁴⁵,Togashi Takashi¹²³⁴⁵,Hasegawa Miki¹²³⁴⁵,Takai Yuki¹²³⁴⁵,Yugi Katsuyuki¹²³⁴⁵,Arakawa Kazuharu¹²³⁴⁵,Iwata Nayuta¹²³⁴⁵,Toya Yoshihiro¹²³⁴⁵,Nakayama Yoichi¹²³⁴⁵,Nishioka Takaaki¹²³⁴⁵,Shimizu Kazuyuki¹²³⁴⁵,Mori Hirotada¹²³⁴⁵,Tomita Masaru¹²³⁴⁵

Affiliation:

1. Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.

2. Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa 252-8520, Japan.

3. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan.

4. Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.

5. Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.

Abstract

Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference22 articles.

1. The model organism as a system: integrating 'omics' data sets

2. Latent Pathway Activation and Increased Pathway Capacity Enable Escherichia coli Adaptation to Loss of Key Metabolic Enzymes

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4. Salt Stress in Desulfovibrio vulgaris Hildenborough: an Integrated Genomics Approach

5. From The Cover: Integration of transcriptomics and metabolomics for understanding of global responses to nutritional stresses in Arabidopsis thaliana

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