Hachimoji DNA and RNA: A genetic system with eight building blocks-Reference-Cited by-同舟云学术

Hachimoji DNA and RNA: A genetic system with eight building blocks

Published:2019-02-22 Issue:6429 Volume:363 Page:884-887
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Hoshika Shuichi¹²^ORCID,Leal Nicole A.¹²^ORCID,Kim Myong-Jung¹²^ORCID,Kim Myong-Sang¹^ORCID,Karalkar Nilesh B.¹²^ORCID,Kim Hyo-Joong¹^ORCID,Bates Alison M.³^ORCID,Watkins Norman E.⁴^ORCID,SantaLucia Holly A.⁴^ORCID,Meyer Adam J.⁵^ORCID,DasGupta Saurja⁶^ORCID,Piccirilli Joseph A.⁶^ORCID,Ellington Andrew D.⁵^ORCID,SantaLucia John⁴^ORCID,Georgiadis Millie M.³^ORCID,Benner Steven A.¹²^ORCID

Affiliation:

1. Firebird Biomolecular Sciences LLC, 13709 Progress Boulevard, No. 17, Alachua, FL 32615, USA.

2. Foundation for Applied Molecular Evolution, 13709 Progress Boulevard, No. 7, Alachua, FL 32615, USA.

3. Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

4. DNA Software Inc., Ann Arbor, MI 48104, USA.

5. Center for Systems and Synthetic Biology, University of Texas, Austin, TX 78703, USA.

6. Department of Biochemistry and Molecular Biology and Department of Chemistry, University of Chicago, Chicago, IL 60637, USA.

Abstract

Expanding the genetic code DNA and RNA are naturally composed of four nucleotide bases that form hydrogen bonds in order to pair. Hoshika et al. added an additional four synthetic nucleotides to produce an eight-letter genetic code and generate so-called hachimoji DNA. Coupled with an engineered T7 RNA polymerase, this expanded DNA alphabet could be transcribed into RNA. Thus, new forms of DNA that add information density to genetic biopolymers can be generated that may be useful for future synthetic biological applications. Science , this issue p. 884

Funder

National Institutes of Health

U.S. Department of Energy

National Aeronautics and Space Administration

Defense Threat Reduction Agency

John Templeton Foundation

Templeton World Charity Foundation, Inc.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference39 articles.