Reconstitution of an intact clock reveals mechanisms of circadian timekeeping

Author:

Chavan Archana G.1ORCID,Swan Jeffrey A.2ORCID,Heisler Joel3ORCID,Sancar Cigdem4ORCID,Ernst Dustin C.4ORCID,Fang Mingxu4ORCID,Palacios Joseph G.2,Spangler Rebecca K.2ORCID,Bagshaw Clive R.2ORCID,Tripathi Sarvind2ORCID,Crosby Priya2ORCID,Golden Susan S.45ORCID,Partch Carrie L.24ORCID,LiWang Andy13467ORCID

Affiliation:

1. School of Natural Sciences, University of California, Merced, CA 95343, USA.

2. Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA.

3. Department of Chemistry and Biochemistry, University of California, Merced, CA 95343, USA.

4. Center for Circadian Biology, University of California, San Diego, La Jolla, CA 92093, USA.

5. Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.

6. Center for Cellular and Biomolecular Machines, University of California, Merced, CA 95343, USA.

7. Health Sciences Research Institute, University of California, Merced, CA 95343, USA.

Abstract

A biological clock in a test tube The biological clock of cyanobacteria, which remarkably requires just three proteins, has been reconstituted in vitro in a system that allows detailed study of its inputs and outputs, bringing new understanding of how environmental signals can influence a biological oscillator and how the clock controls cellular events such as gene transcription. Chavan et al . extended the known in vitro function of the core clock components to include output signals to transcriptional regulation and allow monitoring through fluorescence measurements in real time. The authors combined crystallography, mutagenesis, and quantitative modeling to further explore the clock mechanism, which may enable future synthetic biology applications. —LBR

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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