Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

Author:

Chen Bi-Chang1,Legant Wesley R.1,Wang Kai1,Shao Lin1,Milkie Daniel E.2,Davidson Michael W.3,Janetopoulos Chris4,Wu Xufeng S.5,Hammer John A.5,Liu Zhe1,English Brian P.1,Mimori-Kiyosue Yuko6,Romero Daniel P.7,Ritter Alex T.89,Lippincott-Schwartz Jennifer8,Fritz-Laylin Lillian10,Mullins R. Dyche10,Mitchell Diana M.11,Bembenek Joshua N.11,Reymann Anne-Cecile1213,Böhme Ralph1213,Grill Stephan W.1213,Wang Jennifer T.14,Seydoux Geraldine14,Tulu U. Serdar15,Kiehart Daniel P.15,Betzig Eric1

Affiliation:

1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

2. Coleman Technologies, Incorporated, Newtown Square, PA 19073, USA.

3. National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, FL 32310, USA.

4. Department of Biological Sciences, University of the Sciences, Philadelphia, PA 19104, USA.

5. Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

6. Optical Image Analysis Unit, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.

7. Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA.

8. Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

9. Cambridge Institute for Medical Research, Addenbrooke’s Hospital, Cambridge CB2 0XY, England, UK.

10. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.

11. Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA.

12. Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

13. Max Planck Institute for the Physics of Complex Systems, 01307 Dresden, Germany.

14. Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

15. Department of Biology, Duke University, Durham, NC 27708, USA.

Abstract

From single molecules to embryos in living color Animation defines life, and the three-dimensional (3D) imaging of dynamic biological processes occurring within living specimens is essential to understand life. However, in vivo imaging, especially in 3D, involves inevitable tradeoffs of resolution, speed, and phototoxicity. Chen et al. describe a microscope that can address these concerns. They used a class of nondiffracting beams, known as 2D optical lattices, which spread the excitation energy across the entire field of view while simultaneously eliminating out-of-focus excitation. Lattice light sheets increase the speed of image acquisition and reduce phototoxicity, which expands the range of biological problems that can be investigated. The authors illustrate the power of their approach using 20 distinct biological systems ranging from single-molecule binding kinetics to cell migration and division, immunology, and embryonic development. Science , this issue 10.1126/science.1257998

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Cited by 1544 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3