Affiliation:
1. Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
Abstract
Proteolysis mediated by the anaphase-promoting complex (APC) triggers chromosome segregation and exit from mitosis, yet its regulation is poorly understood. The conserved Cdc20 and Cdh1 proteins were identified as limiting, substrate-specific activators of APC-dependent proteolysis.
CDC20
was required for the degradation of the APC substrate Pds1 but not for that of other APC substrates, such as Clb2 and Ase1. Conversely,
cdh1Δ
mutants were impaired in the degradation of Ase1 and Clb2 but not in that of Pds1. Overexpression of either
CDC20
or
CDH1
was sufficient to induce APC-dependent proteolysis of the appropriate target in stages of the cell cycle in which substrates are normally stable.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
752 articles.
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