Genetic Screens in Human Cells Using the CRISPR-Cas9 System-Reference-Cited by-同舟云学术

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

Published:2014-01-03 Issue:6166 Volume:343 Page:80-84
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Wang Tim¹²³⁴,Wei Jenny J.¹²,Sabatini David M.¹²³⁴⁵,Lander Eric S.¹³⁶

Affiliation:

1. Department of Biology, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.

2. Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.

3. Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA.

4. David H. Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA 02139, USA.

5. Howard Hughes Medical Institute, Department of Biology, MIT, Cambridge, MA 02139, USA.

6. Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Improving Whole-Genome Screens Improved methods are needed for the knockout of individual genes in genome-scale functional screens. Wang et al. (p. 80 , published online 12 December) and Shalem et al. (p. 84 , published online 12 December) used the bacterial CRISPR/Cas9 system to power-screen protocols that avoid several of the pitfalls associated with small interfering RNA (siRNA) screens. Genome editing by these methods completely disrupts target genes, thus avoiding weak signals that can occur when transcript abundance is partially decreased by siRNA. Furthermore, gene targeting by the CRISPR system is more precise and appears to produce substantially fewer off-target effects than existing methods.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference43 articles.

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5. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

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