Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells-Reference-Cited by-同舟云学术

Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

Published:2004-02-06 Issue:5659 Volume:303 Page:832-835
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Boutros Michael¹²³⁴,Kiger Amy A.¹²³⁴,Armknecht Susan¹²³⁴,Kerr Kim¹²³⁴,Hild Marc¹²³⁴,Koch Britta¹²³⁴,Haas Stefan A.¹²³⁴,Consortium Heidelberg Fly Array¹²³⁴,Paro Renato¹²³⁴,Perrimon Norbert¹²³⁴

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

2. Howard Hughes Medical Institute (HHMI), Harvard Medical School, Boston, MA 02115, USA.

3. Zentrum für Molekulare Biologie der Universität Heidelberg, D-69120 Heidelberg, Germany. The Heidelberg Fly Array Consortium consists of Marc Hild, Boris Beckmann, Stefan Haas, Britta Koch, Martin Vingron, Frank Sauer, Jörg Hoheisel, and Renato Paro.

4. Max-Planck Institute for Molecular Genetics, D-14195 Berlin, Germany.

Abstract

A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene ( AML1 ) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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