A Biochemical Genomics Approach for Identifying Genes by the Activity of Their Products-Reference-Cited by-同舟云学术

A Biochemical Genomics Approach for Identifying Genes by the Activity of Their Products

Published:1999-11-05 Issue:5442 Volume:286 Page:1153-1155
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Martzen Mark R.¹,McCraith Stephen M.²,Spinelli Sherry L.¹,Torres Francy M.¹,Fields Stanley²³,Grayhack Elizabeth J.¹,Phizicky Eric M.¹

Affiliation:

1. Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA.

2. Departments of Genetics and Medicine,

3. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Abstract

For the identification of yeast genes specifying biochemical activities, a genomic strategy that is rapid, sensitive, and widely applicable was developed with an array of 6144 individual yeast strains, each containing a different yeast open reading frame (ORF) fused to glutathione S -transferase (GST). For the identification of ORF-associated activities, strains were grown in defined pools, and GST-ORFs were purified. Then, pools were assayed for activities, and active pools were deconvoluted to identify the source strains. Three previously unknown ORF-associated activities were identified with this strategy: a cyclic phosphodiesterase that acts on adenosine diphosphate–ribose 1"-2" cyclic phosphate (Appr>p), an Appr-1"-p–processing activity, and a cytochrome c methyltransferase.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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