Complex scaffold remodeling in plant triterpene biosynthesis-Reference-Cited by-同舟云学术

Complex scaffold remodeling in plant triterpene biosynthesis

Published:2023-01-27 Issue:6630 Volume:379 Page:361-368
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

De La Peña Ricardo¹^ORCID,Hodgson Hannah²^ORCID,Liu Jack Chun-Ting³^ORCID,Stephenson Michael J.⁴^ORCID,Martin Azahara C.⁵^ORCID,Owen Charlotte²^ORCID,Harkess Alex⁶^ORCID,Leebens-Mack Jim⁷^ORCID,Jimenez Luis E.¹^ORCID,Osbourn Anne²^ORCID,Sattely Elizabeth S.¹⁸^ORCID

Affiliation:

1. Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.

2. Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

3. Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

4. School of Chemistry, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.

5. Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

6. HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.

7. Department of Plant Biology, 4505 Miller Plant Sciences, University of Georgia, Athens, GA 30602, USA.

8. Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

Abstract

Triterpenes with complex scaffold modifications are widespread in the plant kingdom. Limonoids are an exemplary family that are responsible for the bitter taste in citrus (e.g., limonin) and the active constituents of neem oil, a widely used bioinsecticide (e.g., azadirachtin). Despite the commercial value of limonoids, a complete biosynthetic route has not been described. We report the discovery of 22 enzymes, including a pair of neofunctionalized sterol isomerases, that catalyze 12 distinct reactions in the total biosynthesis of kihadalactone A and azadirone, products that bear the signature limonoid furan. These results enable access to valuable limonoids and provide a template for discovery and reconstitution of triterpene biosynthetic pathways in plants that require multiple skeletal rearrangements and oxidations.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/science.adf1017

Reference82 articles.

1. Recent progress in the chemistry and biology of limonoids

2. Meliaceous Limonoids: Chemistry and Biological Activities

3. Limonoids: Overview of Significant Bioactive Triterpenes Distributed in Plants Kingdom

4. F. Mulani S. Nandikol H. Thulasiram Chemistry and biology of novel meliaceae limonoids. ChemRxiv 2022-2bpb9 [Preprint] (2022); https://doi.org/10.26434/chemrxiv-2022-2bpb9.10.26434/chemrxiv-2022-2bpb9 2022-2bpb9

5. Research progress of meliaceous limonoids from 2011 to 2021

Cited by 24 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Single-cell RNA-seq based elucidation of the antidepressant hyperforin biosynthesisde novoin St. John’s wort;2024-01-29

2. Progress Toward the Asymmetric De Novo Synthesis of Limonoids;Organic Letters;2024-01-26

3. The evolutionary trajectories of specialized metabolites towards antiviral defense system in plants;Molecular Horticulture;2024-01-12

4. A century of studying plant secondary metabolism—From “what?” to “where, how, and why?”;Plant Physiology;2024-01-02

5. Oxetane Ring Formation in Taxol Biosynthesis Is Catalyzed by a Bifunctional Cytochrome P450 Enzyme;Journal of the American Chemical Society;2023-12-21