Programmable CRISPR-responsive smart materials-Reference-Cited by-同舟云学术

Programmable CRISPR-responsive smart materials

Published:2019-08-23 Issue:6455 Volume:365 Page:780-785
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

English Max A.¹²^ORCID,Soenksen Luis R.²³⁴^ORCID,Gayet Raphael V.¹²⁵^ORCID,de Puig Helena²⁴^ORCID,Angenent-Mari Nicolaas M.¹²⁴^ORCID,Mao Angelo S.²⁴⁶^ORCID,Nguyen Peter Q.⁴⁶^ORCID,Collins James J.¹²⁴⁷⁸^ORCID

Affiliation:

1. Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.

2. Institute for Medical Engineering and Science, MIT, Cambridge, MA 02139, USA.

3. Department of Mechanical Engineering, MIT, Cambridge, Cambridge, MA 02139, USA.

4. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

5. Microbiology Graduate Program, MIT, Cambridge, MA 02139, USA.

6. School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

7. Synthetic Biology Center, MIT, Cambridge, MA 02139, USA.

8. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Abstract

A CRISPR set of materials CRISPR technology is best known as a gene editing tool. English et al. developed a group of stimuli-responsive hydrogels to respond to the programmable nuclease Cas12a (see the Perspective by Han et al. ). The materials undergo molecular to macroscopic changes after Cas12a-dependent cleavage of double- or single-stranded DNA integrated into the gel. The authors show controlled release of particles linked to or imprisoned within the DNA, degradation of a gel with DNA solely forming the cross-links, and permeabilization of a gel with DNA partially forming the cross-links. These tools allow for the production of materials that release encapsulated nanoparticles and cells, act as degradable fuses, and enable remote radio-frequency identification signaling. Science , this issue p. 780 ; see also p. 754

Funder

Defense Threat Reduction Agency

Paul G. Allen Family Foundation

Consejo Nacional de Ciencia y Tecnología

Wyss Institute for Biologically Inspired Engineering

MIT-TATA Center

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference55 articles.

1. CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA