Abstract
Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.
Subject
General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Reference18 articles.
1. Progress with schistosome transgenesis.;Y Alrefaei;Mem Inst Oswaldo Cruz.,2011
2. Transplantation of larval Schistosoma mansoni from infected to uninfected snails.;E Chernin;J Parasitol.,1966
3. Mise en évidence de l’intervention de sporocystes réplicateurs dans le cycle biologique de Schistosoma bovis (Sonsino, 1876) Blanchard, 1895.;C Combes;Bulletin de la Société Française de Parasitologie.,1983
4. Schistosoma haematobium infections acquired in Corsica, France, August 2013.;M Holtfreter;Euro Surveill.,2014
5. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.;M Jinek;Science.,2012
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