Transplantation of schistosome sporocysts between host snails: A video guide

Author:

Mouahid Gabriel,Rognon Anne,de Carvalho Augusto RonaldoORCID,Driguez Patrick,Geyer Kathy,Karinshak ShannonORCID,Luviano NeliaORCID,Mann Victoria,Quack Thomas,Rawlinson Kate,Wendt George,Grunau ChristophORCID,Moné Hélène

Abstract

Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.

Funder

Wellcome Trust

Publisher

F1000 Research Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

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