Serum Amino Acids Changes in adolescent Diagnosed with Kashin-Beck Disease: A Study of Target Metabolomics

Abstract

Abstract Background Kashin-Beck disease ( KBD ) is a chronic degenerative osteoarthropathy with uncertain etiology. This study aims to identify variations in serum amino acids (AAs) between KBD adolescent and control adolescent and to investigate the pathogenesis of the KBD. Method Standard criteria for the diagnosis of KBD (WS/T207-2010) were used to screen children affected by the disease. The study included 31 KBD adolescent and 91 control subjects. The control group consisted of 50 external and 41 internal controls. The serum samples were obtained from the study population and analyzed using Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS). Data were analyzed using the SPSS Statistics 23.0 software. Results Changes in the serum concentrations of 12 different AAs and their metabolites between the KBD children and the control group were detected. Among them, the concentration of 5 AAs (Glutamic acid, Cholamine, Cysteine, Taurine and Asparaginic acid) and their metabolites was either significanlty higher or significanlty lower in the serum of KBD children as compared with the controls (p < 0.05). The serum concentrations of Hydroxyproline, Isoleucine, and Tryptophan between internal and external controls were significanlty different (p < 0.05). The concentrations of Histidine, Threoine, Proline, and 5- hydroxytryptamine among the three groups were significanly different (p < 0.05). Conclusion AAs metabolism changed during the onset of KBD. These findings provide clues into the molecular pathogenesis of KBD.