Evaluation of Antimicrobial Activity of Coleus forskohlii on Periodontal Pathogenic Bacteria, An experimental in-vitro study

Abstract

Abstract Background: Coleus Forskohlii (C.F.) is a typical indigenous medicinal plant from the Lamiaceae family grown widely in India. Coleus gained importance after discovering its therapeutic properties (Bone, 2007) and was reported to have some antibacterial activity. The current study tested the antibacterial effect of (C F) on certain periodontal pathogens. Methods An experimental Invitro-study was conducted at King Khalid University, to test the antibacterial activity of Coleus Forskohlii. Thirty plaque samples were collected from gingivitis patients, kept, and transported using nutrient broth to the microbiology lab. The (CF) extract was prepared from the plant's leaves and five solutions were produced (water, methanol, ethyl acetate, chloroform, and hexane). Following the bacterial culturing, the extract solutions were applied and the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) were measured. Results Various activities were demonstrated, indicating the positive inhibitory reactions of ethanolic extracts against many bacteria grown directly from plaque samples. Twenty-five bacterial species have been inhibited at 1g / mL concentrations. Most organisms (82.9%) have been inhibited at 1g / mL, whereas 34.3% were inhibited at 0.25 g/ mL, and only 5.7% were inhabited at 0.15 g/ mL; the latter two concentrations represented the MIC for most of the strains. Conclusion The ethanolic extract of Coleus forskohlii demonstrated effective antimicrobial activity. The ethanolic extract and water extracts showed different activities against the selected bacteria, but the ethanolic extract was superior to the water extract.