Expression of long non coding RNA H19& miR-675 in colorectal cancer and ulcerative colitis patients

Abstract

Abstract Background: Colorectal cancer (CRC) is a growing public health problem and has been stretching in the recent years from old age group to encompass younger age group. Inflammatory bowel disease like ulcerative colitis is a risk factor for CRC. Aim: Test the expression of microRNA- 675 (miR-675) and long non coding RNA H19 (Lnc H19) for their diagnostic & prognostic potential in CRC. Methodology: A prospective cross-sectional study was conducted on 60 patients with CRC, 60 patients with UC as well as 30 control subjects. miR-675 & lnc H19 were quantified by real time RT-PCR. Lab investigations and radiological investigations were also performed and correlated with the genetic biomarkers. Results: Lnc H19 was highly expressed in both CRC and UC groups with a statistically significant difference from the control group. There was a significant enhanced expression of H19 in CRC compared to UC group. Although miRNA -675 was down regulated in both UC and CRC groups but it was more expressed in CRC compared to UC group. Conclusion: According to our results we can conclude that H19 can be considered as potential non-invasive biomarker for diagnosis of UC and early detection of CRC