Affiliation:
1. Gansu Agricultural University
Abstract
Abstract
Chinese herbal medicine (CHM) has attracted widespread attention due to its natural, non-toxic, and low side-effect properties. Furthermore, Chinese herbal medicines mixture (CHMM) is often considered to have more beneficial effects than a single CHM. The present study was conducted to evaluate the effects of CHMM on antioxidant, immunity and disease resistance against infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss). The results showed that the total superoxide dismutase (T-SOD), catalase (CAT), acid phosphatase (ACP), alkaline phosphatase (AKP) activities of rainbow trout were significantly enhanced (P < 0.05) and the malondialdehyde (MDA) content was significantly reduced (P < 0.05) after feeding with CHMM. Meanwhile, the expression of immune and antiviral related genes (NF-KB, TNF-α, IL-1β, IL-8, MDA5, LGP2, IRF-3, IRF-7, IFN1, JAK1, STAT1 TLR3, TLR7, MYD88 and TGFβ) of rainbow trout were down-regulated after feeding with CHMM. After infected IHNV, the results showed that the all CHMM treatment groups increased Antioxidant and immune-related enzyme activities (T-SOD, CAT, ACP, AKP), while significantly reduced (P < 0.05) the MDA content. The expression of NF-KB, TNF-α, IL-1β, IL-8, MDA5, LGP2, IRF-3, IRF-7, IFN1, JAK1, STAT1, TLR3, TLR7, MYD88 and TGFβ were up-regulated by CHMM. In summary, based on the current experimental conditions, the CHMM has been discovered to effectively improve the antioxidant, immune, and disease resistance capacities of rainbow trout and the recommended dosage of CHMM supplementation for rainbow trout is approximately 30 g/kg.
Publisher
Research Square Platform LLC