Establishing conditions for the generation and maintenance of estrogen receptor-positive organoid models of breast cancer

Author:

Oliphant Michael UJ1,Akshinthala Dipikaa2,Muthuswamy Senthil K2

Affiliation:

1. Harvard Medical School

2. National Cancer Institute, National Institute of Health

Abstract

Abstract Patient-derived organoid models of estrogen receptor-positive (ER+) breast cancer would provide a much-needed tool to understand drug resistance and disease progression better. However, the establishment and long-term maintenance of ER expression, function, and response in vitro remains a significant challenge. Here, we report the development of an ER+ breast tumor organoid medium (BTOM-ER) that conserves ER expression, estrogen responsiveness, and dependence, as well as sensitivity to endocrine therapy of ER+ patient-derived xenograft organoids (PDXO). Our findings demonstrate the utility of subtype-specific culture conditions that better mimic the characteristics of the breast epithelial biology and microenvironment, providing a powerful platform for investigating therapy response and disease progression of ER+ breast cancer.

Publisher

Research Square Platform LLC

Reference25 articles.

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