Affiliation:
1. Wenzhou Medical University
2. Incubation Base for Undergraduates’ Innovation Practice, Naval Medical University
3. Tianjin General Hospital, Tianjin Medical University
4. Naval Medical University
5. Changhai Hospital of Naval Medical University
Abstract
Abstract
Radiation injury is a common side effect of nuclear and radiation accidents as well as clinical oncologic radiotherapy. The organism undergoes a series of pathological responses after irradiation, especially in the short term, accompanied by an intense inflammatory storm [1], and effective targets for intervention have not been identified [2]. In this study, we screened differential genes in gene microarray data from the GEO database and then identified the core gene Itgb1 by enrichment analysis. Subsequently, the expression of Itgb1 was knocked down by siRNA interference and was functionally blocked by RGD, a Itgb1 inhibitor. Next, the proliferation and apoptosis of irradiated cells was detected, and injury of lung tissues and hemopoietic system were also investigated. As a result, knockdown of Itgb1 protected pulmonary epithelial cells and blood cell from irradiation, and RGD remitted the irradiation-induced lung injury and hematopoietic injury. This study suggests that Itgb1 plays a key role in radiation injury and provides new ideas for the prevention and treatment of radiation therapy.
Publisher
Research Square Platform LLC
Reference34 articles.
1. Linking the history of radiation biology to the hallmarks of cancer;Boss MK;Radiat Res,2014
2. Inflammation and chronic oxidative stress in radiation-induced late normal tissue injury: therapeutic implications;Zhao W;Curr Med Chem,2009
3. Research Progress of Epithelial-mesenchymal Transition Treatment and Drug Resistance in Colorectal Cancer;Ni Q;Technol Cancer Res Treat,2022
4. Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry;Obrador E;Antioxidants (Basel),2022
5. CSC Radioresistance: A Therapeutic Challenge to Improve Radiotherapy Effectiveness in Cancer;Olivares-Urbano MA;Cells,2020