Association between CXCL5 and the Coronary Artery Disease and identify the effects of CXCL5 on the adhesion and migration of THP-1 to ECs

Abstract

Abstract Aims: Chemokines play important roles in the development and progression of atherosclerosis and coronary artery disease (CAD). The purpose of this study was to identify the relationship between CXCL5 and the risk of CAD and try to reveal the relative mechanisms. Methods: 395 CAD patientsand 403 controls were enrolled in this study. Using RT-PCR method to detected CXCL5 gene expression and Elisa method to determine CXCL5 plasma levels of were tested by in 160 CAD patients matched well with 160 controls. Established CXCL5over expression, reduction and control EC lines; The chemotactic ability of THP-1 cells to ECs was evaluated by adhesion and transwell assay, and the signaling pathway expression was detected by Western blot. Results: Our study revealed CXCL5 gene expression and serum levels in CAD group were much higher than those in control group (P < 0.01, respectively). CXCL5 concentrations in CAD group (4.85 ± 0.19ng/mL) were significantly higher than that in control group (3.66 ± 0.14ng/mL) (P < 0.05). Logistic regression analysis indicated CXCL5 was a susceptibility factor of CAD and significantly correlated with CAD (P < 0.05). In adhesion and migration experiments, the numbers of THP-1 cells in CXCL5 overexpression groups (EO) were much higher than CXCL5suppression groups (ES), lenti - MOCK infected groups (MOCK) and non - infected groups (EN) (P value < 0.05, respectively). Nevertheless, numbers of THP-1 cells in ES groups were significantly lower than other groups (P < 0.01, respectively). Conclusions: The evidence from our study indicated that CXCL5 might be a new biomarker in assessing the risk of CAD. Our study showed that CXCL5overexpression facilitate the adhesion and migration of THP-1 to ECs.