Comparison of Complete Campylobacter fetus Genomes Derived from Public Repositories

Author:

Paintsil Ellis Kobina1,Adu-Asiamah Cynthia Kyerewaa1,Akenten Charity Wiafe1,Boahen Kennedy Gyau2,Kwarteng Alexander2,Berg Stefan3,Danso Kwasi Obiri2,Dekker Denise3,Ofori Linda Aurelia2

Affiliation:

1. Kumasi Centre for Collaborative Research in Tropical Medicine

2. Kwame Nkrumah University of Science and Technology

3. Bernhard Nocht Institute for Tropical Medicine

Abstract

Abstract Background: Campylobacter fetus(Cf) causes reproductive problems in livestock and infections in immunocompromised individuals. The pathogenesis of Cf can be better understood by using bioinformatics tools to study their genomes. We report on comparative genomic analysis that includes all 23 publicly available complete genomes of C. fetus, as of 30 June 2023, collected from diverse sources across the globe. Results: There was no overlap of ST among the Campylobacter fetussubsp. fetus (Cff), Campylobacter fetus subsp. venerealis(Cfv) and Campylobacter fetus subsp. testudinum (Cft) genomes. The majority (90.0%) of the C. fetus subsp. venerealis (Cfv) were ST-4. Although no phenotypicresistance was predicted, the Bacterial and Viral Bioinformatics Resource Center software identified a total of 635 antibiotic resistance genes, with gyrA, gyrB, MacA and MacB present in all analyzed genomes. One Cff strain (CFF09A980) from bovine harboured tetracycline and streptomycin resistance genes, tet(44) and ant(6)-Ib. AnotherCftstrain (772) isolated from a human host had the tetracycline gene, tet(o). A total of 185 virulence factors were identified from the 23 genomes with each genome having exactly eight virulence factors: flgC, fliQ, fliM, fliN, fliP, fliI, pseB, and cheY. There were 63 prophage elements detected, but only four were intact. There were no protein family exclusive to only one strain. Proteomic analysis showed that Cft strains Sp3 and 772 have the least percentage identity to the reference genome. Phylogenetic tree of the 23 Cf genomes clustered in two major groups; Cft strains clustered together while Cfv, Cfvi and Cff were on one major clade. Conclusion: These findings provide greater insights into the degree of pathogenicity and genomic diversity of complete genomes of Cf.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3