Effects of mouthwash on periodontal pathogens and glycemic control in patients with type 2 diabetes mellitus

Abstract

AbstractPeriodontitis is known to be associated with type 2 diabetes mellitus (T2DM), and gargling with mouthwash is known to reduce the incidence of periodontitis by inhibiting periodontal pathogens. However, the effects of mouthwash on oral and systemic conditions in patients with T2DM remain unknown. In this study, we investigated the effects of gargling with mouthwash on the number of red complex species, includingPorphyromonas gingivalis, Treponema denticola,andTannerella forsythia, and HbA1c levels in patients with T2DM. Patients were instructed to gargle with water for 6 months, followed by gargling with mouthwash containing chlorhexidine gluconate for the subsequent 6 months. At each clinic visit, saliva was collected and bacterial DNA was extracted to detect red complex species using the polymerase chain reaction technique. The HbA1c level was determined using a blood sample. The number of red complex species significantly decreased in younger or male patients while gargling with mouthwash. Furthermore, the number of red complex species significantly decreased in younger patients or patients with higher HbA1c levels while gargling with mouthwash. These results suggest that gargling with mouthwash reduces the number of red complex species and improves hyperglycemic status in patients with T2DM, especially younger patients.