Affiliation:
1. the First Affiliated Hospital of Guangxi Medical University
Abstract
Abstract
Background
Coronary microembolization(CME)is a common complication in acute coronary syndrome and percutaneous coronary intervention, which is closely related to poor prognosis. Studies have shown that colchicine༈COL༉is beneficial in the treatment of coronary artery disease. In addition, pyroptosis was found to be involved in myocardial injury caused by CME. However, the effect of colchicine on myocardial injury caused by CME is unclear. This study was carried out to explore the effects and mechanisms of colchicine on myocardial pyroptosis induced by CME.
Methods
The CME animal model was constructed with Sprague-Dawley rats, and colchicine pretreatment or compound C(CC)co-treatment was given before modeling. The study was divided into 4 groups: Sham group, CME group, CME + COL group, and CME + COL + CC group (10 rats for each group). Cardiac function, serum myocardial injury markers, myocardial histopathology, and pyroptosis-related indicators were used to evaluate the effects of colchicine.
Results
Colchicine pretreatment improved cardiac dysfunction and reduced myocardial injury induced by CME. The main manifestations were the improvement of left ventricular systolic function, the decrease of microinfarction area, and the decrease of mRNA and protein indexes related to pyroptosis. Mechanistically, colchicine increased the phosphorylation level of adenosine monophosphate-activated protein kinase (AMPK), promoted the expression of silent information regulation T1 (SIRT1), and inhibited the expression of NOD-like receptor pyrin containing 3 (NLRP3) to reduce myocardial pyroptosis. However, after CC co-treatment, the effect of colchicine was partially reversed.
Conclusion
Colchicine improves CME-induced cardiac dysfunction and myocardial injury by inhibiting cardiomyocyte pyroptosis through the AMPK/SIRT1/NLRP3 signaling pathway.
Publisher
Research Square Platform LLC