Divergent molecular pathways govern temperature-dependent wheat stem rust resistance genes Sr6, Sr13 and Sr21

Author:

Zhang Peng1ORCID,Hewitt Tim2,Sharma Keshav3,Zhang Jianping4ORCID,Bajgain Prabin5,Bhatt Dhara6,Yang Jun7,Singh Smriti1,Wang Qiaoli8,Upadhyaya Narayana9ORCID,Pozniak Curtis10ORCID,McIntosh Robert1,Lagudah Evans11ORCID,Rouse Matthew12ORCID

Affiliation:

1. University of Sydney

2. Australian National University

3. 6Department of Plant Pathology, University of Minnesota

4. The University of Sydney

5. University of Minnesota

6. CSIRO Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1700, Canberra, ACT 2601, Australia

7. CSIRO Agriculture & Food

8. Henan Agricultural University

9. Commonwealth Scientific and Industrial Research Organisation

10. University of Saskatchewan

11. CSIRO Agriculture and Food, Canberra, ACT

12. USDA-ARS

Abstract

Abstract

The wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), has caused devastating crop losses worldwide. Several stem rust resistance (Sr) genes display temperature-dependent immune responses. Sr6-mediated resistance is enhanced at lower temperatures whereas Sr13 and Sr21 resistances are enhanced at higher temperatures. Here we report cloning of Sr6 by mutagenesis and resistance gene enrichment and sequencing (MutRenSeq), showing it to encode an NLR protein with an integrated BED domain. Sr6 temperature sensitivity was also transferred to wheat plants transformed with the Sr6 transgene. Differential gene expression analysis using near-isogenic wheat lines inoculated with Pgt at varying temperatures revealed that genes upregulated in the low-temperature-effective Sr6 response differed significantly from those upregulated in the high-temperature-effective responses associated with Sr13 and Sr21. Understanding the molecular mechanisms and pathways involved in temperature sensitivity can inform future strategies for deployment and engineering of genetic resistance in response to a changing climate.

Publisher

Springer Science and Business Media LLC

Reference77 articles.

1. McIntosh RA, Wellings CR, Park RF (1995) Wheat Rusts: An Atlas of Resistance Genes. CSIRO Publishing, Melbourne

2. Genetic characterization of stem rust resistance in a global spring wheat germplasm collection;Gao L;Crop Sci,2017

3. Stem rust of small grains and grasses caused by Puccinia graminis;Leonard KJ;Mol Plant Pathol,2005

4. Yield loss associated with different levels of stem rust resistance in bread wheat;Soko T;Plant Dis,2018

5. Roelfs AP (1978) USDA Misc Publ No 1363. U.S. Department of Agriculture, Washington, DC

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