Effect of the oxidative phosphorylation signaling pathway related gene CPT1A on pyroptosis after sevoflurane anesthesia

Abstract

Background Oxidative phosphorylation signaling pathway plays an important role after anesthesia. However, the link between oxidative phosphorylation related genes (OPRGs) and anesthesia is unclear. Methods Transcriptome data was downloaded from the Gene Expression Omnibus (GEO) databases, and 200 OPRGs were obtained from the Molecular Signatures Database (MSigDB). We systematically analyzed the expression profile characteristics of 200 OPRGs and confirmed data quality through tSNE and PCA analysis. Subsequently, the expression level and effect of OPRG carnitine O-palmitoyltransferase 1 (CPT1A) was validated by detected the expression level of inflammation factors. Finally, the activity of pyroptosis pathway was verified after overexpression or knockdown of CPT1A. Results We identified that the expression level of OPRGs were significantly regulated after sevoflurane anesthesia. Compared to the control group, the expression of the inflammatory factors IL-1β, IL-6, and TNF-α were increased. Then, the expression level of CPT1A was up-regulated after sevoflurane anesthesia. In addition, the pyroptosis pathway was activated through after upregulating the expression of inflammatory factors. Conclusion These findings highlight the effect of OPRG CPT1A during anesthesia, deepen the understanding of OPRGs, inflammation, and pyroptosis for patients in clinic anesthesia.