In-vitro evaluation of PCL-based film for guiding segmental bone defect

Author:

Khodabakhshi Vahid1,Soleimanimehr Hamid1,Haghighi Shahram Etemadi1,Emam Ali1

Affiliation:

1. Islamic Azad University

Abstract

Abstract Segmental bone tissue engineering is a highly effective approach for the repair of large bone defects. In this paper, a PCL-based guide film was developed for controlling segmental bone tissue engineering. 3D bioprinting was used to fabricate the PCL/NaCl-based cylindrical films. The effects of the film's thickness and NaCl concentration on the mechanical properties, degradability, swelling behavior, porosity, and cytotoxicity of the samples were investigated. Response surface methodology was employed to study the mechanical behavior using the central composite design (CCD) results showed that increasing the NaCl concentration up to 10% wt. significantly improved the degradability, swelling, and hydrophilicity of the films. It was also indicated that the maximum stiffness of the guide films under vertical loading was almost 5 times more than the maximum stiffness in the horizontal loading direction, but the samples showed greater compressive strength and elongation under horizontal compressive loading. All the evidence indicated that the mechanical properties of the films were more dependent on the film thickness so the thicker films with an 800µm thickness had better mechanical properties in both vertical and horizontal loading. Cytotoxicity assay also approved the non-toxic effect of the PCL films on the MC3T3 osteoblast cell line. Based on the results, the PCL-based films were a suitable candidate to act as a guide for segmental bone tissue engineering.

Publisher

Research Square Platform LLC

Reference32 articles.

1. "Guided bone regeneration with osteoconductive grafts and PDGF: A tissue engineering option for segmental bone defect reconstruction;Alkindi M;Journal of Applied Biomaterials & Functional Materials,2021

2. "Bone tissue engineering techniques, advances, and scaffolds for treatment of bone defects;Alonzo M;Current opinion in biomedical engineering,2021

3. "Investigating the Influence of Architecture and Material Composition of 3D Printed Anatomical Design Scaffolds for Large Bone Defects;Daskalakis E;International Journal of Bioprinting,2021

4. "A modular design strategy to integrate mechanotransduction concepts in scaffold-based bone tissue engineering;Entezari A;Acta Biomaterialia,2020

5. "Porosity and mechanical properties relationship in PCL porous scaffolds;Guarino V;Journal of Applied Biomaterials and Biomechanics,2007

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3