Mechanism of Jinlida regulating microRNA-802 and insulin sensitivity in HepG2 cells exposed to palmitic acid

Abstract

Abstract AIM To explore the effect of Jinlida on microRNA-802 (miR-802) and insulin sensitivity in HepG2 cells interfered with 0.25mmol/L PA. After the modeling, interfered with Jinlida or transfected with miR-802-mimic, the divided experimental groups was as follows: control group (Con), PA group (PA), PA group interfered with Jinlida, Jinlidagroup transfected with miR-802-mimic (miR-802-mimic), Jinlida group transfected with negative control ( NC). The expression level of miR-802 mRNA was detected by RT-PCR methods to determine whether the transfection is successful or not. The expression of protein kinase beta (Akt), phospho-Akt (p-Akt), glycogen synthase kinase -3β(GSK-3β) and phospho- GSK-3β (p- GSK-3β) were determined by western blot. RESULTS Compared with control group, the mRNA expression of miR-802 were significantly increased（P＜0.05）, the protein of p-Akt/Akt and p-GSK-3β/GSK-3βwere significantly decreased（P＜0.05）. Compared with PA group, the mRNA expression of miR-802 was decreased（P＜0.05）, and the protein of p-Akt/Akt and p-GSK-3β/GSK-3βwere increased in PA+JLD group（P＜0.05）. Compared with NC group and PA+JLD group, the mRNA expression of miR-802 was significantly increased（P＜0.05）, and the protein of p-Akt/Akt and p-GSK-3β/GSK-3β were significantly increased in PA+JLD+miR-802-mimic group（P＜0.05）. CONCLUSION JLD might through miR-802 regulating the insulin resistance and glycogen synthesis in the liver, which may be a potential theoretical basis for type 2 diabetes therapy.