Affiliation:
1. Universidade de São Paulo
Abstract
Abstract
Allergic reactions to venom from Hymenoptera insects, such as bees, wasps, and "fire" ants, are relatively common in Brazil, comprising 5-7.5% of all cases of life-threatening anaphylaxis. This study aimed to characterize the activation of the inflammasome in individuals allergic to Hymenoptera venom, considering the established role of the NLRP3 inflammasome in responding to bee venom. We hypothesized that inflammasome activation might be dysregulated in allergic individuals, to this end, inflammasome activation in monocyte-derived macrophages (MDM) isolated from allergic individuals in response to bee, wasp, or "fire" ant venom was evaluated. The MDM of allergic patients displayed heightened responsiveness to the stimuli in comparison to non-allergic individuals, both in terms of IL-1ß release and the rate of pyroptosis. These findings suggest that a pro-inflammatory background could influence the allergic reaction to insect venom in addition to the IgE-related response.
Publisher
Research Square Platform LLC
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