miR-155-5p promotes proliferation, migration, and invasion of osteosarcoma cell line MG-63 through the SOCS1/JAK-STAT1/c-Fos pathway.

Abstract

Abstract [Background] Osteosarcoma (OS) is the most common primary malignant tumor of bone in adolescents, often affecting the limbs and prone to lung metastasis. Therefore, the development of molecular targeted therapy is an exciting approach to improve the prognosis of OS. [Methods] Bioinformatics analysis was conducted to establish the connection between miR-155-5p and the SOCS1/JAK-STAT1/c-Fos pathway. Cell proliferation, migration, and invasion assays were performed to assess the impact of miR-155-5p on MG-63 cells. Transfection of miR-155-5p mimics and inhibitors into MG-63 cells was carried out to examine the expression of miR-155-5p, SOCS1, STAT1, and c-Fos mRNA and protein levels using real-time quantitative PCR and Western blot experiments. [Results] Through bioinformatics analysis and experimental results, we established the following mechanism: upregulation of miR-155-5p in MG-63 cells suppressed the expression of SOCS1, thereby inhibiting the activation of the JAK/STAT1 signaling pathway and further promoting the oncogenic role of c-Fos. In other words, miR-155-5p promotes the proliferation, migration, and invasion of MG-63 cells. The relationship between miR-155-5p and abnormal expression of SOCS1, STAT1, and c-Fos was validated through real-time quantitative PCR and Western blot experiments, confirming that miR-155-5p inhibits the expression of SOCS1 and STAT1 while promoting the expression of c-Fos. [Conclusion] Overexpression of miR-155-5p leads to the suppression of SOCS1 expression, subsequently inhibiting the JAK-STAT1 pathway and promoting the oncogenic role of c-Fos, ultimately contributing to the malignant progression of OS. Therefore, miR-155-5p is considered a potential biomarker and therapeutic target for OS.