Sirt1, as a Potential Prognosis Marker in Renal Clear Cell Carcinoma, regulates lipid metabolism and immune infiltration

Abstract

Abstract Renal clear cell carcinoma (ccRCC) is a malignancy with a dismal prognosis, characterized by the accumulation of fat and glycogen. Sirt1 is a deacetylase that regulates lipid metabolism. In this study, we collected tumor and paracancer tissues from 386 ccRCC patients and followed their prognosis over an extended period. Immunohistochemistry was used to get the expression of Sirt1 in these tissues, and the differential genes associated with Sirt1 were analyzed by the LinkedOmics database. The PPI analysis was made by STRING. The Kaplan-Meier method was utilized to generate the survival curve, while the TIMER web tool was used to analyze immune infiltration. Our findings revealed that Sirt1 was expressed in tumor tissues but not in normal tissues. High Sirt1 expression was associated with poor prognosis. Furthermore, we observed a positive correlation between high Sirt1 expression and perirenal fat invasion and necrosis, which led to worse survival outcomes. We established a nomogram to predict prognosis. Notably, the correlation was observed with immune infiltration. In conclusion, our results suggest that high Sirt1 expression is associated with lipid metabolism disorder and immune infiltration, which in turn contribute to poor prognosis in ccRCC.