EPDRNA: a model for identifying DNA-RNA binding sites in disease-related proteins

Abstract

Abstract Protein-DNA and protein-RNA interactions are involved in many biological processes and essential cellular functions, and most of them are related to disease. To understand the molecular mechanism of protein-DNA binding and protein-RNA binding, it is important to identify which residues in the protein sequence bind to DNA and RNA. At present, there are few methods for specifically identifying the binding sites of disease-related protein-DNA and protein-RNA. In this study, so we developed an ensemble model to predict DNA and RNA binding residues in disease-associated proteins. The data set used in training model was collated from Uniprot and PDB database, and PSSM, physicochemical properties and amino acid type were used as features. The EPDRNA achieved the best AUC value of 0.73 at the DNA binding sites, and the best AUC value of 0.71 at the RNA binding sites in 10-fold cross validation in the training sets. In order to further verify the performance of the model, we did independent test. The EPDRNA achieved 85% recall rate and 25% precision on the protein-DNA interaction independent test set, and achieved 82% recall rate and 27% precision on the protein-RNA interaction independent test set. The online EPDRNA webserver is freely available at http://www.s-bioinformatics.cn/epdrna.