Stilbene Glucoside Ameliorates Symptoms of Attention Deficit Hyperactivity Disorder by Regulating BDNF Signal Pathway and Inhibiting Neuro-inflammation in Spontaneous Hypertensive Rats

Abstract

Abstract Background We investigated the effect of Stilbene on inflammation and the underlying mechanisms in spontaneously hypertensive rats (SHRs). Methods Rats were divided into the control group, the model group, the positive group (4.56 mg/kg/day), Stilbene LD group (40 mg/kg), Stilbene MD group (60 mg/kg), and Stilbene HD group (80 mg/kg). The open field test (OFT) and Morris water maze test (MWM) were used to compare the behavior of the rats among the groups, while real-time polymerase chain reaction (PCR) and western blot were used to compare the expression of cytokines in different brain tissues among the groups. Results OFT and MWM revealed that stilbene significantly reduced hyperactivity and impulsivity, and improved spatial memory in spontaneously hypertensive rats. Stilbene reduced DHA levels in striatum and hippocampus, but increased the mRNA expressions of AKT1, BDNF, SOS1, PIK3CG, GAB1, and NTRK2, and the protein levels of SOS1, GAB1, AKT1, TrkB, and Kinase P110 beta in prefrontal, striatum, and hippocampus. Conclusions Stilbene reduced neuroinflammation and attenuated symptoms of ADHD in SHRs.