Synthesis, characterization, and investigation of pharmacological studies of Some nanoparticles

Abstract

Abstract In this paper, a rapid, facile, and cheap method is used to synthesize gold and titanium dioxide nanogel. Cytotoxicity, invitro anti-inflammatory and antioxidant activities were performed. Invitro antimicrobial activities with molecular docking confirmation were carried out. Also, wound healing was performed in rabbit model with H & E illustration. Characterization and identification of the prepared compounds were investigated using X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and atomic force microscope (AFM). XRD illustrated the presence of the characteristic peaks of the gold and rutile. DLS revealed the homogeneity in size for both gold and rutile nanogel. Where the size of gold/carbopol nanogel and titanium dioxide/carbopol nanogel were 32 nm and 51 nm, respectively, with a zeta-potential of +22 and -28 mV, respectively. BET surface area was found to be 43 and 34 m2/g for gold/carbopol nanogel and titanium dioxide/carbopol nanogel, respectively. The data collected from TEM, SEM, and AFM images studies illustrated the spherical to subspherical shape of gold//carbopol nanogel and titanium dioxide/carbopol nanogel. Cytotoxicity of titanium dioxide/carbopol nanogel and gold/carbopol nanogel on normal human diploid cell line (WI-38 cell) depending on (MTT protocol) and IC50 values were found to be 302.96 and 382.99 ug/ml for titanium dioxide/carbopol nanogel and gold/carbopol nanogel, respectively. Molecular docking with Microbial Beta-Ketoacyl-ACP Synthase II complexed with Malonyl-COA was also presented. The docking score of gold/carbopol nanogel was -3.20 (kcal/mol), however, titanium dioxide/carbopol nanogel was -2.78 (kcal/mol). The anti-microbial effect against ATCC 6538 and ATCC 10221 microorganisms was described and evaluated for the prepared NPs. The MIC50 values recorded were 5 µg/mL and 20 µg/mL for gold/carbopol nanogel and titanium dioxide/carbopol nanogel, respectively. These results confirmed the superior potential antimicrobial effect of gold/carbopol nanogel than titanium dioxide/carbopol nanogel. The antioxidant activity was studied by the DPPH method. The obtained results showed that both synthesized compounds have a potent ability to scavenge hydrogen peroxide when compared to traditional vitamin C. Results of invitro anti-inflammatory activity revealed the potential and superior effect of gold/carbopol nanogel than titanium dioxide/carbopol nanogel. Results of wound healing study revealed the potential and superior efficacy of gold/carbopol nanogel than titanium dioxide/carbopol nanogel.