Affiliation:
1. Shanxi Agricultural University
Abstract
Abstract
Triadimefon, a type of triazole systemic fungicide, has been extensively used to control various fungal diseases. However, extensive application of triadimefon contributes to austere environmental pollution, and even threatens human health. In our study, Enterobacter hormaechei strain TY18 could grew well in carbon salt medium (CSM) with triadimefon as sole nitrogen source, and could significantlydegrade triadimefon. Under triadimefon stress, a total of 430 differentially expressed genes (DEGs), including 197 up-regulated and 233 down-regulated DEGs, were found in strain TY18 from transcriptome sequencing (RNA-Seq). Functional classification and enrichment analysis revealed that these DEGs were mainly related to amino acid transport and metabolism, carbohydrate transport and metabolism, small molecule and pyrimidine metabolism. Interestingly, the DEGs encoding monooxygenase and hydrolase activity acting on carbon-nitrogen were highly up-regulated, might be mainly responsible for the metabolism in triadimefon. The finding in this work suggested that the novel strain E. hormaechei TY18 could degrade efficiently triadimefon for the first time, and contribute to further understanding of the degradation mechanism related to triadimefon in strain TY18.
Publisher
Research Square Platform LLC
Reference46 articles.
1. Pathogen identification and chemical control of powdery mildew on Astragalus membranaceus;Chen TX;Agrochemicals,2013
2. Global transcriptomic analysis of Rhodococcus erythropolis D310-1 in responding to chlorimuron-ethyl;Cheng Y;Ecotox Environ Safe,2018
3. Studies on the chemical control of chrysanthemum white rust caused by Puccinia horiana;Dickens JSW;Plant Pathol,2007
4. Comparative efficacy of myclobutanil, triadimefon and mancozeb against major fungal diseases of chilli;Ganeshan G;Pest Manag Hortic Ecosyst,2011
5. Holt S, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s Manual of Determinative Bacteriology. 9th edn. Lippincott Williams and Wilkins, Baltimore, MD.