Ultrasound-driven nanosilicon transfected tobacco cell as robust factory for collagen III protein synthesis

Abstract

Abstract Background: Therapeutic protein is one of the mostly used medications and the transgenic plant system offers an alternative to synthesis protein with simple practice and reduced infection issues. However, conventional plant transforming vehicles such as agrobacterium and electroporation have been hampered by limited transformation efficiency and biorisk. Methods: Herein, we describe an ultrasound driven nanosilicon composed hybrid-nanoplatform (named as UDSP), which can transform tobacco cells with high efficiency following a simple protocol. Results: With plasmid DNA encoding collagen III gene as a representative, UDSP demonstrates excellent transfection efficiency in tobacco cells with high efficacy to produce collagen III protein, with considerable number of exosomes containing collagen III (Col-Exo) produced simultaneously. The synthesized collagen III protein and Col-Exo are demonstrated for the obvious therapeutic effects in promoting wound regeneration and inflammation alleviation. Conclusions: UDSP transfected tobacco cell is shown as a promising biofactory with efficacy to synthesize recombinant proteins with intact tertiary structure, and therapeutic exosomes with target protein incorporated, with great potential in the protein synthesis and bioengineering.