Cytoskeleton Related Protein KIF9：A Potential Biomarker of Prognosis, 5- Fluorouracil Chemoresistance and Response to Immunotherapy for Patients with Colorectal Cancer

Abstract

Abstract One important clinical predicament and treatment challenge of colorectal cancer (CRC) is chemoresistance of 5-Fluorouracil (5-Fu), affecting the prognosis of patients seriously. The resistant of colorectal cancer to 5-Fu based therapy involves multiple intricate molecular mechanisms and unclear pivotal genes. Kinesin family member 9 (KIF9) is one member of KIFs, a kind of cytoskeleton related protein, which has not been studied in colorectal cancer. In this research, we aimed to explore and elucidate the expression level, the clinical characteristics (age, gender, TNM stage, MSI state, BRAF/P53 mutation) and functions (immune infiltration, prognosis) of KIF9 in colorectal cancer. Furthermore, we found that KIF9 high expression was associated with the response to treatment of 5-Fluorouracil and immunotherapy. The gene and protein expression level of KIF9 was detected by using qRT-PCR and IHC for verification. And we evaluated and predicted the biofunction and pathways of KIF9 in CRC by gene set enrichment analysis. Thus, this article provided a comprehensive and systematic understanding of the biofunctions of KIF9 in colorectal cancer, and we elucidated the role of KIF9 as a biomarker for predicting treatment response of 5-Fluorouracil and immunotherapy.