Anti-inflammatory Effects of Nanoparticles Containing Alpha-Humulene in a Model of Alzheimer's Disease

Abstract

Abstract Alzheimer's disease (AD) is a neurodegenerative disorder that causes impairment in activities of daily living. Although there is no consensus on the pathophysiology, it is known that neuroinflammation is associated with the condition. Objective:To evaluate the anti-inflammatory effects of nanoparticles containing alpha-humulene (HUM) in an experimental model of AD. Methodology: 33 animals were part of the sample, 3 of which were in the negative control group. The other 30 received the amyloid-beta peptide in the CA1 hippocampal region for the neuroinflammation process. They rested for 30 days for the inflammatory process to occur. The HUM group was treated with α-humulene particles, the NHUM with α-humulene nanoparticles for 15 days, and the CP group wasn’t treated. Results:There was a significant difference in IFNconcentration between CP and CN (p=0.0001), HUM and CN (p=0.0003), CP and NHUM (p=0.0006), and HUM and NHUM (p=0.0495) groups. TNF showed no difference between groups. IL-6 showed difference between CP and NHUM (p=0.0078) and CP and CN (p=0.0009). IL-12 showed difference between the CP and CN (p=0.0001) and NHUM and CN (p=0.0160). Among all, the highest concentration was in the CP group. Concerning IL-10, there was a difference between the CP and NHUM (p=0.0003) and NHUM and CN (p=0.0005), with NHUM having the highest concentration. Immunohistochemistry analysis showed in Anti-Tau and Anti-GAPF a strong positive in the CP, a positive in the HUM, and a weak positive in the NHUM group. Conclusion: The Alzheimer's model used and the treatments with HUM and NHUM were effective.